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Fig. 3 | BMC Genomics

Fig. 3

From: Functional characterization of BmOVOs in silkworm, Bombyx mori

Fig. 3

Transcriptional regulatory activity of BmOVO could be regulated by a small peptide encoded by tal-like gene. a Luciferase activities in the co-transfected BmN cells (105) with plasmids pFast-potu5-Luc (1 × 1011 copies), pRL-TK (1 × 1010 copies) and tal-like expression vectors (1 × 1011 copies) were determined at 60 h post-transfection, 100 μg protein from the lysed cells was used for luciferase assay. The co-transfected BmN cells with pFast-potu5-Luc (1 × 1011 copies) and pRL-TK (1 × 1010 copies) plasmids was used as a control. b Plasmids pFast-potu5-Luc-ie1-Bmovo1(1 × 1011 copies), pRL-TK (1 × 1010 copies) and tal-like expression vectors (1 × 1011 copies) were co-transfected into BmN cells (105), and the co-transfected BmN cells with pFast-potu5 (1 × 1011 copies), pRL-TK (1 × 1010 copies) and tal-like expression vectors (1 × 1011 copies) plasmids was used as a control. Luciferase activities were determined at 60 h post-transfection, 100 μg protein from the lysed cells was used for luciferase assay. The ratio of relative luciferase activity means relative luciferase activity in the experimental group than that in the control group. Tal, a full-length cDNA sequence of tal-like; 5A1–4 + B, a sequence containing 5′-non-coding sequence and it’s downstream 1A - 4A with B; A1–4 + B, a sequence containing A1-A4 with B; B, a sequence containing B and its downstream non-coding sequence. (**p < 0.01, ***p < 0.001)

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