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Fig. 5 | BMC Genomics

Fig. 5

From: Functional characterization of BmOVOs in silkworm, Bombyx mori

Fig. 5

Luciferase activity of different truncated Bmotu promoter regulated by BmOVO. a Prediction of cis-acting elements on the promoter of Bmotu (BABH01009636). BmOVO control element (CE) shows as , TATA box as , CAAT box as ▍, primer as →, exon as E, the numbers above the line indicates the size of exon or intron, the number below the line indicate the location of exon in genomic sequence (BABH01009636). b Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO-1. c Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO-2. d Luciferase activity analysis for different truncated Bmotu promoter regulated by BmOVO − 3. BmN cells (105) were transfected with plasmids containing Bmovo expression cassette driven by different truncated otu promoter (1 × 1011 copies) and pRL-TK (1 × 1010 copies). Luciferase activities in the cells were determined at 60 h post-transfection, 100 μg protein from the lysed cells was used for luciferase assay(*p < 0.05,**p < 0.01, ***p < 0.001)

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