Fig. 4

Gene expression patterns obtained with qPCR. Log2 fold changes are expressed as the ratio of gene expression after normalisation to reference genes. Bars represent the means ± SD. Grey, brown trout dark spot; light grey, brown trout light region; red, brown trout red spot; blue, marble trout dark region; light blue, marble trout light region. Expression of each gene was compared with expression in the black spot region of brown trout and statistically evaluated by using unpaired Student’s t-test. A p-value of < 0.05 was considered statistically significant (*p < 0.05; **p < 0.01; ***p < 0.001)