Table 1 Primers used for the qPCR analysis
From: Comparative transcriptome analysis of trout skin pigment cells
Gene symbol | Gene accession no. | Gene description | Forward primer | Reverse primer | Efficiency (%) |
|---|---|---|---|---|---|
rps20 | NM_001140843.1 | 40S ribosomal protein S20 | AGCCGCAACGTCAAGTCT | GTCTTGGTGGGCATACGG | 99.8 |
pgk | XM_014138014.1 | Phosphoglycerate kinase | CTCGGTGATGGGGCTTAGG | TCATTGGTGGAGGCGACA | 98.1 |
dct | XM_014165555.1 | L-dopachrome tautomerase-like | ACATGGCCTGATTCTACAGAGGCTCA | TCAGACCCGAGGGCAGGGC | 110.3 |
mitf | XM_014133193.1 | Microphthalmia-associated transcription factor-like | TGAGGACAGAAGGGGGTCAT | TGCGATTCTGCCATCGTCTT | 93.5 |
tyr | NM_001123643.1 | Tyrosinase | CAGCGGTTCATTGCCTACCT | GTCCGGCCAAACATTTCCTG | 96.4 |
tyrp1 | XM_014206938.1 | Tyrosinase related protein 1b | TGGGAGAACAGTACCAACGC | TGCAAAAAGCGTGCCCTAAC | 99.3 |
pmel | XM_014132714.1 | Melanocyte protein PMEL-like | TTAAGCAGTATCGCCCACTGA | CTGTCGGCTCAGAAGATTCCA | 93.5 |
mc1r | XM_014127040.1 | Melanocortin 1 receptor | ATGCGATCCAGCACCAATGA | GAAGAGCCCAGATGCTCACA | 96.5 |
ednrb | XM_014191488.1 | Endothelin B receptor-like | AGCAATTTAGCGAAGGCGTG | CTGTGGCAGAGGCGTCATAG | 109.7 |
sox10 | XM_014203766.1 | Transcription factor Sox-10-like | TAGCTGGCGGGATTTCCATC | GAGGTGCGGATACTGGTCTG | 99.7 |
aox1 | XM_014151595.1 | Aldehyde oxidase 1-like | AGTGACCAACATGCTCTGGG | ACGCCAACTCTGGAAACACT | 116.2 |
cdkn1a | XM_014166886.1 | Cyclin dependent kinase Inhibitor 1-like | TGGGGCTTTGATTTCCTGTCA | AAAAGGCAGGGTTGGTAGAGG | 106.4 |
wnt10a | XM_014164238.1 | Protein Wnt-10a | GACCCCATTCTCAACGCCAA | TATGCAAAAGCACTCTCACGG | 117.5 |
wnt9b | XM_014204176.1 | Protein Wnt-9b-like | GCCAGAAGAGGGTCAGCAAA | TCGTAGCGGAACTTGAGCAG | 100.2 |
gja1 | XM_014144390.1 | Gap junction alpha-1 protein | TAGTTGCGAACGGAGTTGGT | TGGCACTGGGACATTAAGCC | 87.4 |
gja4 | XM_014178807.1 | Gap junction alpha-4 protein-like | ATAGCCGTGGTTCCAATCCG | GAATGTCACCACCAGGGTCC | 102.5 |
gja5 | XM_014174142.1 | Gap junction alpha-5 protein-like | TGCCTTCTCACCCCTTCAAC | CCGGTATGCAGTAAGGGTGG | 91.2 |
gja9 | XM_014200483.1 | Gap junction alpha-9 protein-like | CGATGCCCGTGTAAAGATGG | CGTGTTGCAGATGAAGTCGG | 112.4 |
gjb1 | XM_014137501.1 | Gap junction beta-1 protein-like | CTGCGATGTTGAGCAGGATG | AAGTGTGACTCGTACCCCTG | 101.5 |
gjb3 | XM_014157241.1 | Gap junction beta-3 protein-like | TGGTACTTCCGCTCACGTTC | AAGTACTCCACTGCGTTCGG | 96.7 |
gjb4 | XM_014143183.1 | Gap junction beta-4 protein-like | TTGCGTGAGGGGAAGGATTC | GACGGGAGATGTAGCAGTCG | 99.7 |
gjd2 | XM_014127494.1 | Gap junction delta-2 protein-like | TTGCACCGGTCGAATCC | AGAGTGTACGGGTAGGGACT | 117.5 |
tjp1 | XM_014170256.1 | Tight junction protein 1 | TATGGCCTTGGTTCGCTGTA | GGTCAGTTCCAAGGCTCACG | 109.8 |
tspan3 | XM_014148589.1 | Tetraspanin 3 | ACACCCTTATCCCTGCTGTG | CCGTAGCGGCTTTCTCGTAT | 99.6 |
slc7a2 | XM_014198602.1 | Cationic amino acid transporter 2-like | GTGTGCTTGCTTCAGCTATCC | GTGCTTGCTGACCAAGTTGT | 89.0 |
slco1c1 | XM_014186199.1 | Solute carrier organic anion transporter family member 1C1-like | AACTTCTTCTGCCGCTTGGA | ACGTTCCTGGGTCTGGTCTA | 92.3 |
kcnc2 | XM_014173424.1 | Potassium voltage-gated channel subfamily C member 2-like | TCCTGACGACCTTTAGGGCA | TGTTGCAGTGGTGTAGCCTT | 94.8 |
clcn2 | XM_014190851.1 | Chloride channel protein 2-like | TTGCTGTATGTTCTCGGGCG | TGGACCTGCCATCGGAATTG | 92.9 |
pcdhac2 | XM_014212430.1 | Protocadherin gamma-C3-like | GGAAGGCTCCGTTGTTGCTA | AGCAAGTGGTCGTTTTGATG | 106.4 |