Fig. 10
Quantitative real-time PCR (qRT-PCR) analysis of DEGs in apple peel after bag removal. a Transcript levels and qRT-PCR results of 15 selected genes from RNA-sequencing. The left y-axis indicates the corresponding RNA-seq data (gray histogram). The right y-axis shows the relative gene expression level measured by qRT-PCR (black lines). The x-axis represents the time (days) after bag removal. Bars represent standard error (n = 3). b Comparison between the log2 values of gene expression ratios obtained from RNA-seq and qRT-PCR methods