Fig. 9

Transcriptional gene silencing of the MdMYB1 gene in tobacco leaves and apple peel. a Glucuronidase (GUS) staining of infiltrated tobacco leaves. b GUS activity of infiltrated tobacco leaves. c GUS staining of the peel of infiltrated fruits. d GUS activity of the peel of infiltrated fruits. e Anthocyanins extracted from the peel of infiltrated fruits. f Concentration of anthocyanins in the peel of infiltrated fruits. All tests were performed in triplicate. pMYB1–2026, infiltrated with pMYB1–2026 (MdMYB1 promoter lacking the − 2026 to − 1870 bp region); pCAMBIA1031–1062, infiltrated with pMYB1–1062 (MdMYB1 promoter lacking the − 1062 to − 964 bp region); pMYB1, infiltrated with pMYB1 (carrying the complete sequence of the MdMYB1 promoter); pCAMBIA1031, infiltrated with pCAMBIA103 (empty vector). Different lowercase letters indicate significant differences by Tukey’s multiple range tests (p < 0.05)