Fig. 5

Proteins involved in cancer-related signaling pathways in P. brassicae and qRT-PCR validation of the expression pattern. a Schematic diagram of proteins encoded by genes of cancer-related signaling pathways in P. brassicae. The black frames represented conserved domains in the genes encoded proteins. The information of conserved domain, e-value, and length was obtained from NCBI database. b Twelve core genes of cancer-related signaling pathways (marked with black solid triangle in (a) were chosen for qRT-PCR validation. Expression levels of these 12 genes from the three different samples (RS, GS and IN) were measured by RNA-seq data (Red line chart) and qRT-PCR data (black histogram). The actin gene of P. brassicae was used as an internal control to normalize the expression level. Data from qRT-PCR represent the means and standard deviations (three replications). R-value of Pearson’s correlation coefficient was used to measure the consistency of the RNA-seq data and qRT-PCR. See Additional file 3: Table S1 and Additional file 4: Table S2 for genes information