Fig. 4

Sequence analysis of the glutathione S-transferase (GST) from S. crosnieri. a Phylogenetic analysis of amino acid sequences of GSTs from S. crosnieri (marked with circles) and those from arthropods using the maximum likelihood method. In the phylogenetic tree, each entry contains the accession number, species name and GST class. Bootstrap values (> 50%) are indicated at branch nodes. b Alignment of amino acid sequences of theta-class GSTs in S. crosnieri with other four arthropods. The N-terminal domain is indicated by the red line; the C-terminal domain is indicated by the blue line; the putative GSH-binding sites (G-sites) are represented by triangles. Double asterisks stand for the amino acids in S. crosnieri with a BEB posterior probability that is larger than 0.99, and one asterisk stands for the sites with a posterior probability higher than 0.95 but lower than 0.99