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Table 1 Summary of the transcriptome data from PacBio RSII platform and Illumina Hiseq platform

From: Comparative transcriptome analyses of genes involved in sulforaphane metabolism at different treatment in Chinese kale using full-length transcriptome sequencing

Platform

Illumina Hiseq (n = 15)

 Raw reads (average)

5,562,602.5

 Clean reads (average)

53,312,258.5

 Clean bases (G, total)

119.97

 Base error rate (average)

0.015%

 Q30 (average)

93.46%

 GC content (average)

47.05%

 Unigenes annotated in at least one database (NR, NT, KO, Swissprot, Pfam, GO, KOG)

129,325

 Unigenes annotated in all databeses

29,191

 Total unigenes

130,553

 TF number

8006 (6.19%)

PacBio RSII (n = 15)

 Total CCS

617,063

 Full length reads

467,672 (75.9%)

 FLNC reads

434,967 (70.5%)

 Average FLNC read length

1720 bp

 Consensus reads

199,106

 Subreads base (G)

13.46

 Subreads number

14,267,901

 Average subreads length

944 bp

 N50

1572

 Total unigenes annotated in at least one database (NR, NT, KOG, Swissprot, Pfam, GO, KEGG)

140,196

 Total unigenes

44,938

 TF number

9407 (5.08%)

  1. CCS circular consensus sequences, FLNC full-length non chimera, GO Gene Ontology, KEGG Kyoto Encyclopedia of Genes and Genomes, KO KEGG Ortholog database, KOG euKaryotic Orthologous Groups, Nr NCBI non-redundant protein sequences, Nt NCBI non-redundant nucleotide sequences, Pfam Protein family, TF transcription factors