Table 3 Genes located in and adjacent to the BIIRfg biosynthetic gene cluster. The predicted function of the genes is based on the in-house sequence annotation pipeline ANNOTATOR. The best blast hit is reported. The observed E-value and percent identity for each gene are shown in parentheses. The rows in bold show the genes thought to be involved in the biosynthesis of BII-Rafflesfungin

BIIRfg_NRPS (QCC62999.1)

Nonribosomal peptide synthase

GAM84991.1

[ Fungal sp. 11,243 ]

(0.0, 43%)

BIIRfg_PKS (QCC63000.1)

Polyketide synthase

ALQ32757.1

[ Fusarium aywerte ]

(0.0, 70%)

orf-a (QCC62998.1)

Thioesterase type II (PF00975)

KPA42378.1

[ Fusarium langsethiae ]

(1e-117, 66%)

orf-b (QCC62997.1)

Hypothetical RTA1 (PF04479) domain containing protein

OQO12260.1

[Rachicladosporium antarcticum]

(3e-133, 60%)

orf-c (QCC62996.1)

Hypothetical protein

OBS28051.1

[Fusarium poae]

(2e-67, 51%)

orf-d (QCC62995.1)

FAD binding domain (PF01565) containing protein

OWY43811.1

[Alternaria alternata]

(0.0, 79%)

orf-e (QCC62994.1)

Similar to heme oxygenase

KPA35830.1

[Fusarium langsethiae]

(2e-85, 63%)

orf-f (QCC62993.1)

MARVEL domain (PF01284) containing protein

EEH09252.1

[Histoplasma capsulatum]

(3e-58, 56%)

orf-g (QCC62992.1)

Putative membrane transport protein (Major facilitator superfamily, PF07690)

KXL45980.1

[Acidomyces richmondensis]

(0.0, 54%)

orf-h (QCC62991.1)

Glycosyltransferase (PF03033)

EEH09251.1

[Histoplasma capsulatum]

(0.0, 70%)

orf-i (QCC62990.1)

AMP dependent ligase (PF00501)

KPA42376.1

[ Fusarium lansethiae ]

(0.0,69%)

orf-j (QCC62989.1)

Hypothetical P-loop containing ABC transporter protein

OKO93750.1

[Penicillium subrubescens]

(0.0, 34%)

orf-k (QCC62988.1)

Heme binding (PF00141)

KZM26722.1

[Ascochyta rabiei]

(0.0, 89%)