Fig. 3
Rearrangements in rDNA-bearing chromosomes among F. pratensis × L. perenne hybrids. (a, b) F2 2A-33 with recombined 3L/non-rDNA-bearing Festuca chromosome; (c, d) F2 2B-21 with recombined 2F chromosome; (e, f) F2 2B-28 with recombined one of 1, 2, 7L group of chromosomes, and (g, h) F2 2A-5 without 2F chromosomes. (a, c, e, g) FISH with 5S rDNA (red) and 35S rDNA (green); chromosomes were counterstained with DAPI (blue). (b, d, f, h) GISH with total genomic DNA of L. perenne as a probe (green) and with blocking genomic DNA of F. pratensis (orange) on the same metaphase plate; chromosomes were counterstained with propidium iodide (orange). Red dotted lines indicate the place of rearrangement. Chromosomes with rearrangements corresponding with rDNA position were exemplified in the insets. Scale bar 5 μm