Fig. 4

Differentially expressed miRNAs between libraries. a Differentially expressed miRNAs between S15 and D15. b Differentially expressed miRNAs between S20 and D20. c Differentially expressed miRNAs between S30 and D30. d Heatmap of differentially expressed miRNAs. e Distribution of differentially expressed miRNAs. P values were transformed to negative log₁₀ values, and fold changes of miRNA expression between libraries were transformed to log₂ values. Negative log₁₀ P values and log₂-fold changes are shown on the y- and x-axes, respectively. The green spots indicate downregulated miRNAs, the red spots indicate upregulated miRNAs, and the blue spots indicate miRNAs whose expression did not evidently change between the libraries. The data are presented as log₂ (fold change) values comparing miRNA abundances (TPM) between D15 and S15, D20 and S20, and D30 and S30