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Fig. 5 | BMC Genomics

Fig. 5

From: Integrated microRNA and transcriptome profiling reveals a miRNA-mediated regulatory network of embryo abortion under calcium deficiency in peanut (Arachis hypogaea L.)

Fig. 5

Quantitative RT-PCR validation of differentially expressed miRNAs and their corresponding target genes under calcium deficiency and sufficiency. The yellow bar represents relative changes in expression levels, as calculated by the 2-CT method; qRT-PCR analysis was used to analyze the differentially expressed miRNAs. The orange line indicates relative changes in expression levels for differentially expressed miRNAs, as determined by RNA-seq. The blue bars represent changes in relative expression levels of target genes, as determined by qRT-PCR. The purple line indicates changes in relative expression levels of target genes, as calculated by the FPKM using RNA-seq. All qRT-PCRs and RNA-seq analyses were performed for three biological replicates

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