Genome-wide RAD sequencing to identify a sex-specific marker in Chinese giant salamander Andrias davidianus

BMC Genomics

Table 3 Female-specific primers

Primer	Sequence (5′-3′)	Annealing temperature (°C)	Product size (bp)
adf225s	CCATGCCCTGTACATTTGCG	59.899	162
adf225a	CCGTGAACATGGAGGGGTTT	60.251
adf340s	TTAACGGCCCTAACACCAGG	59.674	251
adf340a	GGTTTAGGGCGGCTCTGATT	60.107
adf318a	TATGTCAGGGTGATCAAACTCTTCA	59.5	266
adf318s	CTAGAAGACGTGGTGGCCATG	60.0
adf431a	TCCAGAATGAAGTCCTGGCCT	59.1	178
adf431s	CGAGCCTCCATTGTGCCTT	59.8

ISSN: 1471-2164