Ribosomal transcripts are enriched in uORF datasets. a Top: Chart shows the percent of total actively translated ribosomal protein transcripts (n = 70) with a predicted uORF (n = 59). Bottom: Chart shows the percent of ribosomal transcripts with uORFs that are in the highly translated dataset (n = 23). Ribosomal transcripts are enriched in both sets by Fisher’s exact test, ****p > 0.0001. b nLuc assay in SH-SY5Y cells transfected with uORF reporters for ribosomal transcripts: RPS8 and RPS18. c Immunoblot of RPS8 and RPS18 reporters show an increase in molecular weight relative to AUG-nLuc control, confirming translation initiation within the 5′ leader of these ribosomal transcripts. d Removal of the 5′ leader portion of the RPS24 uORF from nLuc reporters for the RPS24 CDS increased nLuc signal relative to reporters with the WT RPS24 5′ leader.