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Table 2 Percentage of fragments mapped to individual milk protein transcripts in relation to the total number of fragments in each sample as counted by featureCounts [50]

From: Development and evaluation of a milk protein transcript depletion method for differential transcriptome analysis in mammary gland tissue

Udder quarter treatment

Animal No.

Sample treatmenta

LALBA

CSN1S1

CSN3

CSN2

CSN1S2

PAEP

Non-challenged

Cow1

0

2.7

15.7

11.5

31.1

4.2

5.5

A

3.1

17.9

0.6

9.6

0.3

4.5

B

2.6

12.2

0.2

8.7

0.2

5.9

Cow2

0

0.8

18.7

9.6

14.3

4.9

3.9

A

0.5

18.7

0.8

3.1

0.1

2.2

B

0.4

16.4

0.2

2.8

0.1

2.0

Cow3

0

1.0

16.7

9.5

21.6

4.9

7.4

A

1.0

14.3

0.7

4.2

0.1

5.2

B

0.8

11.6

0.1

3.8

0.1

5.0

Challenged

Cow1

0

1.1

15.2

11.1

21.9

3.3

4.3

A

1.0

12.1

0.6

5.9

0.1

3.8

B

0.9

9.0

0.2

5.4

0.0

4.0

Cow2

0

0.4

12.3

7.5

6.3

2.1

4.2

A

0.2

6.2

0.2

2.2

0.0

1.8

B

0.2

4.5

0.0

1.4

0.0

1.7

Cow3

0

0.0

0.1

0.5

0.1

0.0

0.0

A

0.0

0.1

0.0

0.0

0.0

0.0

B

0.0

0.0

0.1

0.0

0.0

0.0

  1. aUdder tissue samples without RNA depletion (0), with RNA depletion by variant A (A) or variant B (B). Variant A and B differ in antisense oligonucleotide input for depletion (see Materials and Methods)