Fig. 5From: Trans-chalcone activity against Trichophyton rubrum relies on an interplay between signaling pathways related to cell wall integrity and fatty acid metabolismWestern blot results of Mapk expression and Mapk phosphorylation. Trans-chalcone was added or not (control) to the medium for 1âh, 1âday, and 3âdays. Antiphospho-p44/42MAPK antibody was used to detect MpkA phosphorylation. The Îł-tubulin antibody was used as control (a). Gene expression analysis of CMGC/MAPK Erk1 in T. rubrum by qPCR. The fungus was grown in medium with trans-chalcone (0.24âÎŒg/mL) or without the compound (control) for 1âh, 1âday, and 3âdays (b)Back to article page