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Fig. 2 | BMC Genomics

Fig. 2

From: Identification of genes underlying phenotypic plasticity of wing size via insulin signaling pathway by network-based analysis in Sogatella furcifera

Fig. 2

Flight muscle related genes are highly expressed and alternatively spliced in MFW. a The volcano plot of the muscle-related genes’ expression profiles. Plotted along the x-axis is log2 fold-change of average gene expression levels measured by FPKM values between MFW and BFW, and along the y-axis is the negative logarithm of the p-values with base 10. Red circles represent 20 up-regulated genes with P-value < 0.05 and log2 fold change > 1. The horizontal dot line is the negative logarithm of the P-value threshold (P = 0.05). The vertical dot line is the log2 fold-change of 1. b The volcano plot of all the genes’ differential exon usage. Plotted along the x-axis is the log2 fold-change of relative exon usage between MFW and BFW, and along the y-axis is the negative logarithm of the Benjamini-Hochberg (BH) adjusted p-values with base 10. Red points are significantly alternatively spliced exons (ASEs) with BH adjusted P-value < 0.05 and fold change > 2. The red triangles indicate ASEs in the troponin I gene. The horizontal red dot line is the negative logarithm of the BH adjusted P-value threshold (P = 0.05). The vertical black dot lines are log2 fold-change of − 1 and 1, at the left and right, respectively. c Expression levels of each exon of troponin I. Red denotes BFW and blue MFW. Beneath the plot is the gene model of troponin I. d The longer and shorter isoforms of troponin I transcripts were verified by RT-PCR. Arrowheads indicate the positions of PCR primers. The exons in yellow represent the common exons between the two isoforms, while the exons in purple represent the exclusive exons of the longer isoform. The primer set of P1 and P3 was used for the longer isoform, while the P1 and P2 set for the shorter isoform

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