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Fig. 7 | BMC Genomics

Fig. 7

From: Transcriptional analysis of renal dopamine-mediated Na+ homeostasis response to environmental salinity stress in Scatophagus argus

Fig. 7

Changes in NKAα1 expression and NKA activity in S. argus kidneys during salinity tolerance experiments. a Immunolocalization of NKAα1 in S. argus kidney. NKAα1 was visualized using a primary antibody against NKAα1 (green, b), and nuclei were identified by staining with DAPI (blue, a and d). Negative control was incubated with normal rabbit serum (e). Scale bar = 25 μm. b NKA activity in kidneys isolated from fish acclimated to different salinities. c Expression profile of nkaα1 from RNA-Seq (pink) and RT-qPCR (blue) analyses of changes in transcript expression following salinity acclimation. β-actin served as a reference gene. The results are presented as the mean ± SEM with three replicates. d Western blot analysis of NKAα1 and β-actin in S. argus kidney during salinity stress

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