Fig. 2

The molecular basis of O, H antigen formula for Sendai, Miami and Paratyphi A. a A frameshift mutation occurred in rfbE in the entire lineage of Paratyphi A, but not in Sendai. Consequently, Paratyphi A needs to recruit a different start codon to initiate translation. b Seven different peptides were detected for RfbE in Paratyphi A by proteomic analysis (see details in Additional File 2), with their positions marked by black boxes. The position of possible start codons (ATG) were marked by red arrows. c Incongruent phylogeny between fliB, fliC and fliD. For fliC, Miami is nearly identical to Paratyphi A and Sendai; but for fliB and fliD, Miami is relatively remote. SPA1, S. Paratyphi A ATCC 9150; SPA2, S. Paratyphi A ATCC 11511; SEND, S. Sendai BAA1672; SMI, S. Miami BAA1586; SPAN, S. Panama ATCC 7378; STM, S. Typhimurium 14028S; SCH, S. Choleraesuis SCB67; SPC, S. Paratyphi C RKS4594; STY, S. Typhi Ty2; SDU, S. Dublin CT_02021853; SPUL, S. Pullorum CDC1983–67; SGA, S. Gallinarum 287/91; SENT, S. Enteritis P125109