Fig. 1
Identification of Xa21 mutant lines in the T0 generation. a PCR-detected presence and absence of individual transgenes (Hpt and Cas9) in T0 transgenic plants; 1–2 were T0 transgenic-negative lines, and 3–13 were T0 transgenic-positive lines. b Susceptible phenotype of T0 transgenic lines 3 to 6. c Sequence alignment of the sgRNA target region showing the altered bases in T0 transgenic lines 3 to 6. + 2: 2 bp insertion. -10, 10 bp deletion; + 1, 1 bp insertion; − 3, 3 bp deletion; WT, Xa21 wild type. The sgRNA-targeted site is denoted with a dotted line, and the PAM sequence is in black bold font