Fig. 2From: Application of high-throughput amplicon sequencing-based SSR genotyping in genetic background screeningResistance performance and expression of Xa21 in mutants. a Susceptible phenotype of the T1 plants. b Expression levels of Xa21 in T1 plants and wild-type DXT as detected by qPCR. Asterisks indicate a significant difference compared to the DXT line at P < 0.05 with Student’s t-test. c XA21 protein levels in T1 plants and wild-type plants as detected by western blot analysis. 3–1 and 3–2, Xa21 mutant lines (T1 plants of line 3); DXT, wild-type line (Xa21 transgenic line); DXB, Xa21 MAB-produced line; rubisco, the control for total leaf proteinBack to article page