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Table 1 Phenotypes and mutation patterns of the 11 T0 transgenic-positive lines

From: Application of high-throughput amplicon sequencing-based SSR genotyping in genetic background screening

T0 line

T0 generation

T1 segregation ratio

T-DNA

Phenotype

Zygosity

Genotype

R

S

Total

3

+

S

Com-He

-10, + 2

0

30

30

4

+

S

Ho

+ 1, + 1

0

30

30

5

+

S

Ho

-3, −3

0

20

20

6

+

S

Ho

+ 1, + 1

0

20

20

7

+

R

He

–

3

17

20

8

+

R

He

–

10

10

20

9

+

R

He

–

7

12

19

10

+

R

He

–

7

33

40

11

+

R

He

–

10

15

25

  1. R resistant, S susceptible, Ho homozygote, He Heterozygote, Com-he compound heterozygote. -10, 10-bp deletion; + 2, 2-bp insertion; + 1, 1-bp insertion; −3, 3-bp deletion; −, genotype not tested