TY - JOUR AU - Sharma, Ashok Kumar AU - Pafčo, Barbora AU - Vlčková, Klára AU - Červená, Barbora AU - Kreisinger, Jakub AU - Davison, Samuel AU - Beeri, Karen AU - Fuh, Terence AU - Leigh, Steven R. AU - Burns, Michael B. AU - Blekhman, Ran AU - Petrželková, Klára J. AU - Gomez, Andres PY - 2019 DA - 2019/06/14 TI - Mapping gastrointestinal gene expression patterns in wild primates and humans via fecal RNA-seq JO - BMC Genomics SP - 493 VL - 20 IS - 1 AB - Limited accessibility to intestinal epithelial tissue in wild animals and humans makes it challenging to study patterns of intestinal gene regulation, and hence to monitor physiological status and health in field conditions. To explore solutions to this limitation, we have used a noninvasive approach via fecal RNA-seq, for the quantification of gene expression markers in gastrointestinal cells of free-range primates and a forager human population. Thus, a combination of poly(A) mRNA enrichment and rRNA depletion methods was used in tandem with RNA-seq to quantify and compare gastrointestinal gene expression patterns in fecal samples of wild Gorilla gorilla gorilla (n = 9) and BaAka hunter-gatherers (n = 10) from The Dzanga Sangha Protected Areas, Central African Republic. SN - 1471-2164 UR - https://doi.org/10.1186/s12864-019-5813-z DO - 10.1186/s12864-019-5813-z ID - Sharma2019 ER -