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Fig. 5 | BMC Genomics

Fig. 5

From: mRNA profiling reveals significant transcriptional differences between a multipotent progenitor and its differentiated sister

Fig. 5

Reporter expression validates differential gene expression. (a) Previously published gene reporters show expression of ehn-3, pes-1 fkh-6, lag-2, tra-1, cyd-1, dsh-2, lin-26, sys-1, pop-1, ztf-16, and dgn-1 in SGPs (red) and bgal-1 and arg-1 (blue) in hmcs. We detected expression of all of these genes in our dataset (not shown). log2[fold-change] in expression between SGPs and hmcs is reported. Positive numbers indicate higher expression in SGPs (red bars); Negative numbers indicate higher expression in hmcs (blue bars). (b) The R151.2 locus produces at least eight transcripts from four promoters. The C. elegans gene expression consortium generated an R151.2 transcriptional reporter (BC15463). The 2932 bp genomic region used to drive reporter expression in BC15463 is shown; it includes only three of the four known promoters. We created an endogenous R151.2 reporter by using CRISPR/Cas9 to insert the viral T2A peptide upstream of GFP coding sequences and immediately before the R151.2 stop codon. All previously described R151.2 transcripts contain the last exon of the gene; therefore this reporter is predicted to reflect the expression of all R151.2 isoforms. (c) The BC15463 reporter is expressed in intestine and cells of the head and tail, but not in SGPs at the L1 larval stage. (d) The R151.2::T2A::GFP reporter is expressed in intestine, cells in the head and tail, and in SGPs at the L1 larval stage. (c-d) GFP expression is shown for the whole worm (top). DIC (C′-D’) and GFP fluorescence (C“-D”) are shown for higher magnification images of the gonad primordium (bottom). White boxes indicate the area of magnification. Arrows point to SGPs (only one SGP is visible in C′)

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