Fig. 5

Comparison of RNA extraction and library preparation protocols. a Left panel: Hierarchical clustering based on 100 most differentially detected mature miRNAs sequences (≥10 reads from 550,000 reads mapping to miRNAs). Right panel: Correlation matrix of pairwise comparisons of miRNA expression between all RNA extraction/library preparation combinations. b Left panel: Hierarchical clustering based on 500 most differentially detected miRNA isomiR sequences (≥2 reads from 550,000 reads mapping to miRNAs). Right panel: Correlation matrix of pairwise comparisons of isomiR expression between all RNA extraction/library preparation combinations. The three main clusters in the Left panels and blocks in the Right panels demonstrate the similarity between libraries of the same type and are subdivided according to RNA extraction method