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Table 3 The immune responses related DEGs and partial validation of RNA sequencing data by qRT-PCR

From: Transcriptome analysis reveals differential immune related genes expression in bovine viral diarrhea virus-2 infected goat peripheral blood mononuclear cells (PBMCs)

Gene ID

Gene name

Function annotation

FC log2(B2/N)

RNA-Seq

qRT-PCR

Down-regulated

 NC_030809.1_gene90

C1QA

complement C1q subcomponent subunit A

−3.03

 

 NC_030809.1_gene93

C1QB

complement C1q subcomponent subunit B

−2.29

 

 NC_030809.1_gene91

C1QC

complement C1q subcomponent subunit C

−1.42

 

 NC_030814.1_gene546

CFD

complement factor D

−2.35

 

 NC_030812.1_gene428

S-LE

lysozyme

−3.03

 

 NC_030836.1_gene709

IFITM3

interferon-induced transmembrane protein 3

−1.36

 

 NC_030834.1_gene266

LOC102188015

beta-defensin 103A

−1.05

−0.5

 NC_030810.1_gene1245

S100A4

protein S100-A4

−1.76

 

 NC_030808.1_gene1009

S100B

protein S100-B

−1.23

 

 NC_030818.1_gene1111

FCN1

ficolin-1

−1.56

 

 NC_030818.1_gene586

ANXA4

annexin A4

−1.54

−0.7

 NC_030813.1_gene593

ANXA3

annexin A3

−1.49

−0.9

 NC_030817.1_gene452

ANXA2

annexin A2

−1.29

−1.9

 NC_030826.1_gene194

CCL2

C-C motif chemokine 2

−1.61

−2.2

Up-regulated

 NC_030828.1_gene424

NFKBIA

NF-kappa-B inhibitor alpha

1.27

1.3

 NC_030836.1_gene157

LOC102168428

serum amyloid A protein

1.31

2.2

 NC_030836.1_gene161

LOC100860781

serum amyloid A3

1.62

 

 NC_030826.1_gene156

CCL3

C-C motif chemokine 3

1.48

1.5

 NC_030825.1_gene308

CCL22

C-C motif chemokine 22

1.48

 

 NC_030826.1_gene155

CCL4

C-C motif chemokine 4

1.95

1.8

 NC_030826.1_gene163

CCL5

C-C motif chemokine 5

1.80

1.6

 NC_030809.1_gene360

CCL20

C-C motif chemokine 20

3.12

2.7

 NC_030813.1_gene567

CXCL10

C-X-C motif chemokine 10

1.16

1.2

 NC_030814.1_gene1059

ICAM1

intercellular adhesion molecule 1

1.54

 

 NC_030830.1_gene594

TNF

tumor necrosis factor alpha

1.62

3

 NC_030823.1_gene84

IL-10

interleukin-10

2.16

2.5

 NC_030830.1_gene686

IL-17A

interleukin-17A

2.18

1.6

 NC_030811.1_gene808

IL-6

interleukin-6

2.28

2.4

 NC_030814.1_gene174

IL-12B

interleukin-12 subunit beta

2.80

2.2

 NC_030814.1_gene828

GM-CSF

granulocyte-macrophage colony-stimulating factor

3.30

2.45