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Table 2 The essence of the altered “cumulome” after maturation in vitro

From: Analysis of the equine “cumulome” reveals major metabolic aberrations after maturation in vitro

Metabolic Groups with their Proteins/Metabolites Abbreviation Figure Proteomics: Orthologous Human Accession Uniprot Proteomics: NCBI Accession Anova (Progenesis) Max fold change (Progenesis) Up/Down in-vitro
Metabolomics: HMDB ID Metabolomics: Compound (m/z)
1 Oxygen Supply (proteins: n = 5; metabolites n = 2)
 Hemoglobin A HBA HBA_HUMAN NP_001078901.1 0.015 25.58 Down
 Hemoglobin B HBB HBB_HUMAN NP_001157490.1;XP_001504241.1 0.007 52.65 Down
 Ceruloplasmin CP CERU_HUMAN XP_001491539.1;XP_014587394.1;XP_014587022.1 < 0.001 140.94 Down
 Transferrin TF TRFE_HUMAN NP_001075415.2 0.003 10.25 Down
 Hemopexin HEMO HEMO_HUMAN XP_005612174.1 0.010 10.14 Down
 Hydroxymethylbilane Hydroxymethylbilane HMDB01137 4.89_426.1165 m/z 0.001 16.86 Down
 Bilirubin-Glucuronide Bilirubin-Gluc HMDB10332 6.37_741.2851 m/z 0.040 2.21 Up
2 Glucose Metabolism (proteins n = 4; metabolites n = 5)
 Glutamine-fructose-6-phosphate aminotransferase 1 GFPT1 GFPT1_HUMAN XP_005599972.1 < 0.001 101.18 Down
 Glutamine-fructose-6-phosphate aminotransferase 2 GFPT2 GFPT2_HUMAN XP_014585826.1 0.024 2.80 Down
 ATP dependend 6-phosphofructokinase muscle type PFK-M PFKAM_HUMAN XP_005611133.1;XP_014596234.1 0.006 4.67 Up
 Solute Carrier Family 2, facilitated glucose transporter member 1 GLUT1 GTR1_HUMAN NP_001157443.1;XP_005607060.1 0.040 2.01 Down
 Phosphoenolpyruvate PEP HMDB00263 6.23_166.9749 m/z 0.018 157.36 Up
 D-Lactate / L-Lactate Lactate HMDB00190 6.41_111.0085 m/z 0.019 2.78 Up
 Hexose-Monophosphate Hexose-P not specified 6.41_259.0217 m/z 0.039 6.10 Up
 Citrate/Isocitrate Citrate HMDB00094 6.84_191.0191 m/z 0.024 4.04 Up
 2-phospho-d-glyceric acid 2PG HMDB03391 6.39_185.9927n 0.026 10.30 Up
3 Fatty Acid Metabolism (proteins n = 9; metabolites n = 3)
 Apolipoprotein A1 APOA1 APOA1_HUMAN XP_005611649.1 0.001 30.68 Down
 Apolipoprotein A2 APOA2 APOA2_HUMAN XP_001503846.1 0.003 48.44 Down
 Apolipoprotein H APOH APOH_HUMAN XP_001499728.1 < 0.001 30.74 Down
 Adiponectin ADIPO ADIPO_HUMAN XP_001499564.1 0.009 8.64 Down
 Acetyl-CoA carboxylase 1 ACACA ACACA_HUMAN XP_005597614.1;XP_001496980.1 < 0.001 6.07 Up
 Fatty acid synthase FASN FAS_HUMAN XP_014591306.1 0.001 2.64 Up
 Long-chain-fatty-acid-CoA ligase 3 ACSL ACSL3_HUMAN XP_001915556.1 0.013 4.14 Up
 Fatty acid binding protein FABP FABPH_HUMAN NP_001157357.1;XP_005607059.1 0.001 46.96 Up
 Acyl-CoA desaturase ACOD ACOD_HUMAN XP_001500414.1 < 0.001 7.97 Up
 O-Phosphoethanolamine O-Phosphoethanolamine HMDB00224 4.64_175.9943 m/z 0.002 2.76 Up
 Diacyglycerol (40:7) DAG (40:7) not specified 3.61_647.5261 m/z 0.024 11.18 Up
 CDP-Ethanolamine CDP-Ethanolamine HMDB01564 4.85_222.0249 m/z 0.021 2.75 Up
4 Oxidative Phosporylation (proteins n = 1; metabolites n = 1)
 NADH-ubiquinone oxidoreductase chain 5 Mt-ND5 NU5M_HUMAN NP_007170.1 0.046 3.61 Up
 reduced flavin mononucleotide FMNH2 HMDB01142 7.19_439.0852 m/z < 0.001 4.37 Up
5 Amino Acid Metabolism (proteins n = 7; metabolites n = 4)
 phosphoserine aminotransferase PSAT1 SERC_HUMAN XP_001496412.1 0.022 9.40 Up
 L-Serine; D-Serine SERINE HMDB00187; HMDB03406 4.98_104.0352 m/z 0.008 3.22 Down
 Phosphohydroxypyruvic acid PHPA HMDB01024 6.82_164.9577 m/z 0.000 19.00 Up
 Leucine/Isoleucine L; Iso-L HMDB00687; HMDB00172 6.55_168.0429 m/z 0.003 7.84 Up
 L-Cystine Cystine HMDB00192 4.81_221.0093 m/z 0.008 2.67 Up
 Serine t-RNA ligase SARS SYSC_HUMAN XP_005610429.1 0.007 2.17 Up
 Tyrosine t-RNA ligase YARS SYYC_HUMAN XP_014592779.1 0.015 2.06 Up
 Asparaginyl t-RNA ligase NARS SYNC_HUMAN XP_001488269.1 0.016 3.19 Up
 Threonine t-RNA ligase TARS SYTC_HUMAN XP_001498225.2;XP_001491149.3 0.005 2.11 Up
 Methionine t-RNA ligase MARS SYMC_HUMAN XP_001488941.1;XP_005611399.1 0.039 2.12 Up
 Alanine t-RNA ligase AARS SYAC_HUMAN XP_001501062.3 0.013 34.61 Down
6 Purin & Pyrimidin Metabolism (proteins n = 8; metabolites n = 3)
 Ribose-phosphate pyrophosphokinase PRPS1 PRPS1_HUMAN XP_001491425.1;XP_001489096.2;XP_001496502.1 0.011 2.09 Up
 Phosphoribosylformylglycinamidine synthase PFAS PUR4_HUMAN XP_001918417.1 0.006 14.09 Up
 Bifunctional purine biosynthesis protein PURH PUR_9_HUMAN XP_005610644.2 0.007 2.01 Up
 Inosine-5′-monophosphate dehydrogenase 2 IMDH2 IMDH2_HUMAN XP_001494600.4;XP_005600741.2 0.004 3.17 Up
 Guanine Monophosphate Synthase GMPS GUAA_HUMAN XP_014587031.1 0.003 2.23 Up
 DNA-directed RNA polymerases I, II, and III subunit RPABC1 POLR2 RPAB1_HUMAN XP_001496313.1;XP_014596768.1 0.026 3.93 Up
 Bis(5′-adenosyl)-triphosphatase ENPP4 ENPP4_HUMAN XP_001502639.1 0.005 5.49 Down
 Hypoxanthine HypoX HMDB00157 4.75_172.9976 m/z 0.014 2.62 Up
 Guanosine Guano HMDB00133 4.63_318.0790 m/z 0.033 4.29 Up
 Deoxycytidine monophosphate dCMP HMDB01202 6.89_343.9941 m/z 0.025 2.25 Up
 Uridine 5′-monophosphate synthase UMPS UMPS_HUMAN XP_001500089.4 0.009 3.17 Up
7 Steroid Metabolism (proteins n = 2; metabolites n = 2)
 Diphosphomevalonate decarboxylase MVD MVD1_HUMAN XP_001488083.1;XP_014593934.1 0.007 6.21 Up
 Growth Regulation by Estrogen in Breast Cancer Gene 1 GREB GREB1_HUMAN XP_014586792.1 0.002 4.26 Up
 Geranyl Pyrophosphate Geranyl-PP HMDB01285 4.89_314.0589n 0.002 3.04 Down
 Estrone Sulfate Estrone-S HMDB01425 6.98_385.0719 m/z < 0.001 2.94 Down
8 Extracellular Matrix/Proteoglycans (proteins n = 11; metabolites n = 1)
 Vitronectin VN VTNC_HUMAN XP_001504173.3 0.001 19.83 Down
 Fibronectin FN FINC_HUMAN XP_001489154.4 < 0.001 152.88 Down
 Inter-alpha-trypsin inhibitor heavy chain H1 ITIH1 ITIH1_HUMAN XP_001492576.1;XP_014587106.1 < 0.001 76.91 Down
 Inter-alpha-trypsin inhibitor heavy chain H2 ITIH2 ITIH2_HUMAN XP_001916967.1 < 0.001 45.40 Down
 Inter-alpha-trypsin inhibitor heavy chain H3 ITIH3 ITIH3_HUMAN XP_014587105.1 < 0.001 14.74 Down
 Inter-alpha-trypsin inhibitor heavy chain H4 ITIH4 ITIH4_HUMAN XP_005600608.1;XP_014587103.1 0.011 26.91 Down
 Basement-membrane specific heparan sulfate proteoglycan core protein HSPG2 PGBM_HUMAN XP_014592730.1 0.014 3.17 Down
 Epidermal Growth Factor Receptor EGFR EGFR_HUMAN XP_005609210.2 0.009 2.24 Up
 Dystroglycan DAG1 DAG1_HUMAN XP_001497663.1;XP_005600719.1;XP_005600720.1;XP_005600721.1 0.011 2.35 Up
 Serine Protease HTRA1 HTRA1 HTRA1_HUMAN XP_005602601.1 0.013 3.92 Up
 Gap junction alpha 1 protein; Connexin-43 GJA1 CXA1_HUMAN NP_001296155.1;XP_014584258.1;XP_014584259.1 0.008 2.69 Down
 Chondroitin-4-sulphate CHOND4 HMDB00652 4.75_496.0430 m/z 0.012 3.61 Up
9 Complement Cascade (proteins n = 14)
 Plasma protease C1 inhibitor IC1 IC1_HUMAN; SERPING1 XP_001498388.1 < 0.001 56.92 Down
 Complement 4 (A/B) C4 CO4B_HUMAN XP_001492943.1 0.007 7.78 Down
 C4b-binding protein alpha chain C4BP C4BPA_HUMAN XP_014594908.1 0.001 48.62 Down
 C4b-binding protein alpha chain C4BP C4BPA_HUMAN XP_001492582.2;XP_005609671.1;XP_005609672.1;XP_005609676.1;XP_005609677.1;XP_005609678.1;XP_014594955.1;XP_014594956.1;XP_014594957.1;XP_014594958.1 < 0.001 73.54 Down
 C3 = Complement C3 C3 CO3_HUMAN XP_014596564.1 0.005 9274.92 Down
 C3 = Complement C3 C3 CO3_HUMAN XP_001915589.1 0.001 748.41 Down
 Complement C5 C5 CO5_HUMAN XP_014591698.1;zz|FGCZCont0049_P01031|CO5_HUMAN 0.044 5.66 Down
 Complement C7 C7 CO7_HUMAN XP_005604353.1 0.004 33.92 Down
 Complement factor I CFI CFAI_HUMAN XP_014593434.1;XP_014593435.1;XP_014593436.1 < 0.001 16.51 Down
 Complement factor B CFB CFAB_HUMAN XP_001492602.1 0.008 33.85 Down
 Complement factor H X1 CFH CFAH_HUMAN XP_001491754.3 0.019 70.12 Down
 Complement factor H X5 CFH CFAH_HUMAN XP_005608148.2 0.002 12.74 Down
 Clusterin CLUS CLUS_HUMAN NP_001075413.1 0.049 3.44 Down
 Vitronectin VN VTNC_HUMAN XP_001504173.3 0.001 19.83 Down
10 Coagulation Cascade (proteins n = 17)
 Fibrinogen alpha chain FGA FIBA_HUMAN XP_005607860.1 0.001 43.25 Down
 Fibrinogen beta chain FGB FIBB_HUMAN XP_003364583.1 < 0.001 55.46 Down
 Fibrinogen gamma chain FGG FIBG_HUMAN XP_001914833.2 < 0.001 36.00 Down
 Kininogen-1 KNG KNG1_HUMAN XP_001499389.1;XP_005601930.1 0.008 14.66 Down
 Prothrombin F2 THRB_HUMAN XP_001490892.3 0.001 6.66 Down
 Heparin Cofactor 2 HEP2 HEP2_HUMAN; SERPIND1 XP_003365492.1 0.003 46.20 Down
 Plasma serine protease inhibitor IPSP IPSP_HUMAN; SERPINA5 XP_001496026.2 0.031 44.21 Down
 Antithrombin 3 ANT3 ANT3_HUMAN; SERPINC1 XP_014594947.1;zz|FGCZCont0237_P01008|ANT3_HUMAN 0.001 8.46 Down
 Plasma protease C1 inhibitor IC1 IC1_HUMAN; SERPING1 XP_001498388.1 <0.001 55.27 Down
 Alpha-2-antiplasmin A2AP A2AP_HUMAN; SERPINF2 XP_001504386.3;XP_005597710.1 0.005 10.84 Down
 Alpha-2-macroglobulin A2M A2MG_HUMAN XP_014596179.1 0.015 49.65 Down
 Alpha-2-macroglobulin A2M A2MG_HUMAN XP_014596181.1;XP_014596182.1 0.014 40.92 Down
 Alpha-2-macroglobulin A2M A2MG_HUMAN XP_001499173.2 0.000 15.00 Down
 Plasminogen PLG PLMN_HUMAN XP_001500552.3 0.027 16.17 Down
 Alpha-1-antiproteinase 2 precursor A1AT A1AT_HUMAN NP_001108005.1 0.001 35.00 Down
 Alpha-1-antiproteinase 2 precursor A1AT A1AT_HUMAN XP_005605481.1 0.001 33.45 Down
 Alpha-1-antiproteinase 2 precursor A1AT A1AT_HUMAN XP_001495905.2 0.001 27.21 Down