Fig. 3

Phylogenetic relationship and gene structure of PYL family in three Nicotiana species. The unrooted neighbor-joining (NJ) evolutionary tree was constructed using MEGA 7.0 with 1000 bootstrap replicates based on the deduced full-length amino acid sequences of the PYLs in Nicotiana tabacum, N. tomentosiformis, and N. sylvestris (left panel). Exon-intron analyses of the identical Nicotiana PYL genes were performed with GSDS 2.0 (right panel). Exons and introns are represented by black rectangle and black lines, respectively. The lengths of the exons and introns for each Nicotiana PYL gene are shown proportionally