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Table 2 Occurrence of chimeric BC–ROI combinations under different PCR conditions for high-diversity MPRA libraries

From: Optimized PCR conditions minimizing the formation of chimeric DNA molecules from MPRA plasmid libraries

Type of PCR Elongation time, sa MPRA library Total read count Reads with chimeric BC–ROI combinations, %
name sizeb R#1 R#2 R#1 R#2
emulsion 10 library-71 22,621 526,056 497,217 0.580 0.555
30 library-71 51,650 594,511 514,941 0.342 0.310
30 library-83 38,156 371,889 456,380 0.261 0.304
conventional 30 library-71 85,249 776,878 784,138 0.336 0.337
30 library-83 52,654 788,801 809,402 0.295 0.310
  1. aIndicated elongation time was used in both rounds of PCR
  2. bThe number of genuine BCs found in both replicates
  3. R#1 and R#2 stand for replicate 1 and replicate 2, respectively