Representative Figure(s) | Assessment | Clontech | Illumina | NEB | NEXTflex | Deduped | Fivepercent |
---|---|---|---|---|---|---|---|
Method information | |||||||
 Figure 1 | Designed for adapter ligation mitigation? | Adapter Ligation free | None | PEG | PEG and Randomized adapters | PEG and Randomized adapters | PEG and Randomized adapters |
 Figure 1 | Designed for reverse transcription and PCR bias mitigation? | None | None | None | None | UMI | None |
Performance metrics | |||||||
 Similarity | |||||||
 Figure 2 | How similar are the quantifications of the same brain sample? | lowest correlations with other methods | Intermediate | Intermediate | Intermediate | Intermediate | Intermediate |
 Accuracy | |||||||
 Figure 3 | Accuracy - similar detection of different sequences | Intermediate | Worst | Worst | Intermediate | Best | Intermediate |
Detection Diversity | |||||||
 Figure 4a-b | Mapping rate to miRNA | Worst | Best | Best | Best | Besta | Besta |
 Figure 4c | Detection of miRNA across triplicates at 1000 ng | Intermediate | Intermediate | Intermediate | Intermediate | Best | Worst |
 Figure 4d | Consistency of miRNA detection at 1000 ng | Worst | Intermediate | Best | Intermediate | Intermediate | Intermediate |
 Figure 4e | Diversity of detection of isomiRs across triplicates at 1000 ng | Best | Intermediate | Intermediate | Worst | Intermediate | Worst |
 Figure 4f | Consistency of isomiR detection at 1000 ng | Intermediate | Worst | Best | Intermediate | Intermediate | Intermediate |
 Figure 4i | False isomiR detection per synthetic sequence | Worst | Intermediate | Intermediate | Best | Best | Best |
 Figure 4j | False IsomiR expression | Intermediate | Intermediate | Worst | Best | Best | Best |
Consistency | |||||||
 Figure 5a | Consistency across batch | Intermediate | Worst | Best | Best | Best | Intermediate |
 Figure 5b-c | Consistency across triplicates | Intermediate | Worst | Best | Intermediate | Best | Worst |
Overall conclusions | |||||||
 |  | Poor mapping rate, more accurate miRNA quantification, poor isomiR accuracy | Not as accurate, less detection, and less consistency | Good mapping, not as accurate, quite consistent, poor isomiR accuracy | Good mapping, more accurate miRNA quantification, more accurate isomiR quantification | Best performance overall, more accurate miRNA quantification | Deduping improves performance, not just an effect of reducing number of reads |