Table 3 Primers used for RT-PCR in the present study
From: Dynamic DNA methylation of ovaries during pubertal transition in gilts
Name | Sequence | Product(bp) | Accession number |
|---|---|---|---|
DNMT1 | F: GGGAGATGTGGAGATGCTATGC | 271 | NM_001032355.1 |
R: TGCTGGTACTGAAGCGGTTG | |||
DNMT3a | F: GCGAAGTGAGGACCATTACTACGA | 126 | NM_001097437.1 |
R: AGCCGAACACCCTTTCCATCT | |||
DNMT3b | F: GAGCGTAGAAGCAAGGAAACAC | 332 | NM_001348900.1 |
R: CTGGCAGCCGTCATCGTCATA | |||
ESR1 | F:GATGCCTTGGTCTGGGTGAT R:AGTGTTCCGTGCCCTTGTTA | 124 | NM_214220.1 |
FGF21 | F:CACGAAACTGAAGCCCACCTG R:TTGTAGCCATCCTCAAGAAGC | 165 | NM_001163410.1 |
GSK3B | F:AACCACCTTCTTTGCGGAG R:GCTTGGCTTGATACACGACA | 214 | NM_001128443.1 |
IGF1R | F:AGAACTGCACGGTGATCGAG R:AGATGACCAGGGCGTAGTTG | 178 | NM_214172.1 |
TAC3 | R:GACTTCTTTGTGGGTCTTATGG F:GCAGTTTCTACAGACGGTGG | 283 | NM_001007196.1 |
MMP2 | R:GATGGCTTCCTTTGGTGTTCC R:CTTCTTGTCGCTGTCGTAGTCC | 318 | NM_214192.2 |
GAPDH | F:TCCCGCCAACATCAAAT R:CACGCCCATCACAAACAT | 253 | NM_001206359.1 |