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Fig. 2 | BMC Genomics

Fig. 2

From: A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells

Fig. 2

Transposon mutagenesis drives vemurafenib resistance in A375 cells. Cells were engineered as described in Fig. 1. Twenty-four hours after mutagenesis was initiated, 10 cm plates were seeded with mutagenized or control cells. After allowing cells to attach, 5 μM vemurafenib was added to the culture medium. Culture media and drug were replaced twice weekly until colonies emerged on the plates containing mutagenized cells. Colonies were stained with Coomassie Blue. Representative plates are shown

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