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Fig. 3 | BMC Genomics

Fig. 3

From: A simplified transposon mutagenesis method to perform phenotypic forward genetic screens in cultured cells

Fig. 3

Comparison of transposon insertion sites in a series of vemurafenib-resistant A375 colonies. Eight distinct vemurafenib-resistant colonies were isolated from three independent populations of SB-mutagenized A375 cells. Each colony was expanded and transposon insertion sites were identified (see Fig. 1). Between 10 and 135 insertion events were present in each expanded colony. In general, transposon insertion sites were distributed across the genome, suggesting that transposon mutagenesis is capable of performing genome-wide screens

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