Carriage of Shiga toxin phage profoundly affects Escherichia coli gene expression and carbon source utilization

BMC Genomics

Table 1 Growth characteristics of MG1655 and the Stx2 lysogens in minimal medium supplemented with singe carbon sources

Strain	Growth medium^a	OD = 0.4 pre-culture		ON pre-culture
Strain	Growth medium^a	Generation time [min]^b	Final OD₅₉₅^b	Generation time [min]^b	Final OD₅₉₅^b
MG1655	MM glucose	88 ± 3	0.41 ± 0.00	81 ± 2	0.39 ± 0.01
MG::ϕO104		103 ± 1***	0.31 ± 0.01***	106 ± 3***	0.33 ± 0.02**
MG::ϕPA8		106 ± 2***	0.35 ± 0.00***	101 ± 6**	0.34 ± 0.01**
MG1655	MM maltose	120 ± 3	0.34 ± 0.00	111 ± 1	0.32 ± 0.00
MG::ϕO104		187 ± 13***	0.23 ± 0.01***	184 ± 5***	0.15 ± 0.00***
MG::ϕPA8		206 ± 4***	0.24 ± 0.00***	200 ± 9***	0.18 ± 0.01***
MG1655	MM L-lactate	175 ± 1	0.25 ± 0.02	170 ± 1	0.29 ± 0.01
MG::ϕO104		295 ± 43**	0.10 ± 0.01***	949 ± 20***	0.05 ± 0.00***
MG::ϕPA8		218 ± 7	0.09 ± 0.00***	526 ± 19***	0.05 ± 0.01***
MG1655	MM galactose	172 ± 5	0.38 ± 0.00	166 ± 2	0.35 ± 0.00
MG::ϕO104		193 ± 12*	0.16 ± 0.00***	254 ± 10**	0.09 ± 0.00***
MG::ϕPA8		224 ± 1**	0.10 ± 0.01***	319 ± 27***	0.07 ± 0.00***
MG1655	MM sialic acid	102 ± 2	0.44 ± 0.00	91 ± 0	0.43 ± 0.00
MG::ϕO104		108 ± 1**	0.30 ± 0.00**	109 ± 2***	0.32 ± 0.00***
MG::ϕPA8		105 ± 1	0.30 ± 0.00**	114 ± 3***	0.33 ± 0.00***
MG1655	MM ribose	180 ± 4	0.35 ± 0.00	177 ± 4	0.29 ± 0.00
MG::ϕO104		456 ± 9***	0.03 ± 0.00***	457 ± 46***	0.02 ± 0.00***
MG::ϕPA8		791 ± 61***	0.02 ± 0.01***	583 ± 29***	0.02 ± 0.00***
MG1655	LB	32 ± 1	0.97 ± 0.02	33 ± 1	0.94 ± 0.00
MG::ϕO104		33 ± 0	0.12 ± 0.01***	33 ± 1	0.12 ± 0.01***
MG::ϕPA8		32 ± 1	0.28 ± 0.02***	34 ± 1	0.27 ± 0.00***

*The strains displaying significantly different growth characteristics in comparison to MG1655 are marked: * p < 0.05, ** p < 0.01, *** p < 0.001
^aCells were grown either in minimal medium (MM) supplemented with 0.2% of single carbon source or in the nutrient rich LB medium
^bThe values represent mean and standard error of the mean of three biological replicates

ISSN: 1471-2164