Fig. 1
A simple method to isolate germ nuclei from C. elegans. a Schematic representation of the method to isolate germ nuclei from C. elegans. Approximately 1 × 106 young adult hermaphrodites were collected for nuclei isolation for each experiment. For RNA-seq, worms were homogenized with 15 loose and 15–22 tight Dounce strokes after collection. For ChIP-seq, worms were fixed with 2% formaldehyde for 28 min before homogenization with 15 loose and 22–30 tight Dounce strokes (see Materials and Methods). A typical yield is 1.5–3 × 107 nuclei from 1 × 106 young adult hermaphrodites. b Representative image of isolated total nuclei from young adult worms stained with DAPI. Scale bar, 10 μm