Fig. 3
Expression profiling of C. elegans isolated germline nuclei. RNA from two independent replicates of wild type IGN and from SOM (glp-1(q224) young adults) was analyzed for expression profiling. a Volcano plot showing –log10 of q-value against log2 of fold change for each gene. The number of genes that were significantly up-regulated in SOM or IGN are indicated. Black line marks the significance cutoff of q = 0.05 (Y axis). Blue circles indicate SOM-enriched transcripts and red circles indicate IGN-enriched transcripts. b The most significant Gene Ontology Biological Process terms of the 5075 IGN-enriched transcripts. c-d Bar graphs indicating expected and observed number of genes (Y axis) in different gene categories [22] (X axis) for IGN-enriched transcripts (c) and SOM-enriched transcripts (d). Asterisks indicate significantly more genes than expected (hypergeometric test, p-value< 1 × 10− 5 [**], p-value< 1 × 10− 10 [***]). e Overlap of 4831 IGN-enriched coding transcripts with previously-identified germline-expressed transcripts in dissected gonads [23]. f The ratio of IGN FPKM to dissected gonad FPKM for genes previously defined as having soma-specific, germline-specific, and ubiquitous expression [24]