Fig. 2

Sex determining genes expression at adult stage in P. perniciosus. The first lane left is 100-bp ladder (NEB). a Positive RT-PCR control with Ppesod+/Ppesod-primer pairs. These PCR primers span a 112-bp long intron of Ppesod gene (genomic amplicon size 450 bp; cDNA amplicon size 338 bp). b Ppetra-2 RT-PCR amplification. c Ppedsx RT-PCR amplification. d Ppefru RT-PCR amplification. Light green boxes represent non-sex specific untranslated regions. Dark green boxes represent non-sex-specific coding regions. Azure boxes represent male-specific untranslated regions. Pink boxes represent female-specific untranslated regions. Blue and red boxes represent male-specific and female-specific coding regions, respectively. Grey boxes represent fru alternative terminal exons encoding for Zinc Finger domains. The position of primers utilized for each gene are indicated by short red arrows. Yellow vertical bars indicate the position of the putative TRA/TRA-2 binding sites. e Putative TRA/TRA-2 binding sites identified in Ppedsx and Ppefru female-specific transcripts