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Fig. 7 | BMC Genomics

Fig. 7

From: Identification of sex determination genes and their evolution in Phlebotominae sand flies (Diptera, Nematocera)

Fig. 7

Phylogenetic relationship and protein sequence comparison of TRA/FEM proteins. a TRA/FEM protein schemes were aligned using the conserved sex-specific splicing site located within the TRACAM domain encoding region as reference point (indicated by the red triangle). This sex-specific splicing site is conserved in all the autoregulating tra genes. In sand flies TRA, the TRACAM domain is absent. Striped yellow-grey box represents the position of the homologous sex-specific splicing site in P. perniciosus TRA. D. melanogaster TRA protein was aligned using the position of the non-conserved sex-specific splicing site. The crustacean Daphnia magna TRA was aligned using the position of the conserved TRACAM domain. Percentages within red and blue boxes indicates the percentage of R and S residues and of P residues in the RS and Proline-rich domains, respectively. To define the boundaries of the RS domain, we considered the position of the first RS or SR couple of residues till the final RS or SR couple of residues and we considered the selected region an RS domain only if its percentage of R and S is > of 25%. b Multiple alignment of insect TRACAM domains and the P. perniciosus TRA homologous region. Amino acids conserved in at least two species are highlighted in black. The conserved sex-specific splicing site is indicated by red triangle

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