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Table 3 Absolute quantitative analysis and their expression of the BBTV genomic components of B2 and B4 samples

From: Independent modulation of individual genomic component transcription and a cis-acting element related to high transcriptional activity in a multipartite DNA virus

Isolate Component DNA Ct(dRn) DNA Aver. (copies/μg) RNA Ct(dRn) RNA Aver. (copies/μg) RNA/DNA ratios (%)
1 2 3 1 2 3
B2 DNA-R 23.93 23.99 24.12 0.50 26.14 26.05 25.92 0.48 95.92
DNA-U3 21.44 21.51 21.58 2.97 22.71 22.71 22.53 5.52 185.79
DNA-S 23.61 23.85 23.71 0.79 23.78 23.84 23.72 3.03 384.35
DNA-M 22.08 22.01 21.98 1.98 24.32 24.41 24.17 1.69 85.45
DNA-C 24.91 24.8 24.82 0.24 27.05 27.07 26.92 0.22 91.62
DNA-N 22.09 21.99 22.11 2.33 20.7 20.68 20.53 24.67 1059.44
S2 21.54 21.49 21.6 3.40 24.36 24.42 24.37 2.01 59.26
Sat4 21.99 21.99 21.84 2.92 24.65 24.64 24.54 1.77 60.71
B4 DNA-R 24.83 24.91 24.92 0.27 27.23 27.16 26.93 0.23 83.78
DNA-U3 22.13 22.27 22.37 1.80 23.55 23.67 23.50 4.81 267.49
DNA-S 23.77 23.97 23.83 0.72 24.15 24.23 24.23 2.28 316.14
DNA-M 22.07 22.11 22.07 1.90 24.42 24.37 24.27 1.63 85.74
DNA-C 25.01 25.07 25.13 0.21 27.17 27.11 26.97 0.21 101.96
DNA-N 21.57 21.69 21.41 3.30 20.47 20.33 20.25 30.01 910.09
Sat4 21.39 21.27 21.25 4.57 24.33 24.41 24.34 2.11 46.21