Table 3 Absolute quantitative analysis and their expression of the BBTV genomic components of B2 and B4 samples
Isolate | Component | DNA Ct(dRn) | DNA Aver. (copies/μg) | RNA Ct(dRn) | RNA Aver. (copies/μg) | RNA/DNA ratios (%) | ||||
|---|---|---|---|---|---|---|---|---|---|---|
1 | 2 | 3 | 1 | 2 | 3 | |||||
B2 | DNA-R | 23.93 | 23.99 | 24.12 | 0.50 | 26.14 | 26.05 | 25.92 | 0.48 | 95.92 |
DNA-U3 | 21.44 | 21.51 | 21.58 | 2.97 | 22.71 | 22.71 | 22.53 | 5.52 | 185.79 | |
DNA-S | 23.61 | 23.85 | 23.71 | 0.79 | 23.78 | 23.84 | 23.72 | 3.03 | 384.35 | |
DNA-M | 22.08 | 22.01 | 21.98 | 1.98 | 24.32 | 24.41 | 24.17 | 1.69 | 85.45 | |
DNA-C | 24.91 | 24.8 | 24.82 | 0.24 | 27.05 | 27.07 | 26.92 | 0.22 | 91.62 | |
DNA-N | 22.09 | 21.99 | 22.11 | 2.33 | 20.7 | 20.68 | 20.53 | 24.67 | 1059.44 | |
S2 | 21.54 | 21.49 | 21.6 | 3.40 | 24.36 | 24.42 | 24.37 | 2.01 | 59.26 | |
Sat4 | 21.99 | 21.99 | 21.84 | 2.92 | 24.65 | 24.64 | 24.54 | 1.77 | 60.71 | |
B4 | DNA-R | 24.83 | 24.91 | 24.92 | 0.27 | 27.23 | 27.16 | 26.93 | 0.23 | 83.78 |
DNA-U3 | 22.13 | 22.27 | 22.37 | 1.80 | 23.55 | 23.67 | 23.50 | 4.81 | 267.49 | |
DNA-S | 23.77 | 23.97 | 23.83 | 0.72 | 24.15 | 24.23 | 24.23 | 2.28 | 316.14 | |
DNA-M | 22.07 | 22.11 | 22.07 | 1.90 | 24.42 | 24.37 | 24.27 | 1.63 | 85.74 | |
DNA-C | 25.01 | 25.07 | 25.13 | 0.21 | 27.17 | 27.11 | 26.97 | 0.21 | 101.96 | |
DNA-N | 21.57 | 21.69 | 21.41 | 3.30 | 20.47 | 20.33 | 20.25 | 30.01 | 910.09 | |
Sat4 | 21.39 | 21.27 | 21.25 | 4.57 | 24.33 | 24.41 | 24.34 | 2.11 | 46.21 | |