Fig. 10

Diagram illustrating the rearing and infection of Scaphoideus titanus and the transmission trials. Hatched larvae (first instar), obtained from wood material collected from a very infested vineyard, were divided into two groups: the first was fed with grapevine leaves collected from FD-healthy grapevine plants, the second was fed with fresh FD-infected leaves from field-grown grapevine plants. For the transmission trials two S. titanus specimens (fifth instars or adults), both FD-free (HSt) or infective (FDSt), were put on every micropropagated plantlet pair and were allowed to feed for three days. Half of the plantlets were collected after three days (3 dpi), while the remaining were kept until six days (6 dpi). The asterisks indicate the points at which PCR analyses for FD phytoplasma presence were done