Fig. 4

A large majority of Olfr mRNAs shows multiple 3’UTR isoforms produced through alternative polyadenylation. The whole male_IS2104 dataset was analyzed using our IsoSCM pipeline. a Distribution of numbers of 3’UTR isoforms per Olfr. b Distribution of length for 3’UTR groups; sUTR = single 3’UTR for Olfr genes without APA (purple); pUTR = proximal 3’UTR isoform (dark green) and dUTR = distal 3’UTR isoform(s) (light green) for Olfr genes with APA. One-way Kruskal-Wallis test (Chi square = 430.62, p < 0.0001, df = 2), followed by Nemenyi test (* p < 0.05, *** p < 0.001). c Distribution of Olfr genes in 4 distinct quantitative 3’UTR isoform profiles. P1 = Olfr genes without APA (their mRNAs present a single 3’UTR), P2 = Olfr genes with APA, the proximal 3’UTR isoform representing more than 80% of the mRNAs (typically, the proximal 3’UTR is the major isoform); P3 = Olfr genes with APA, the proximal 3’UTR isoform representing less than 80% and the sum of the most distal isoforms (dUTR2 to dUTR4) less than 10% (typically, the pUTR and dUTR1 are the main isoforms); P4 = Olfr genes with APA, the proximal 3’UTR isoform representing less than 80% and the sum of the most distal isoforms (dUTR2 to dUTR4) more than 10% (typically, these Olfr genes have 3 or more isoforms of quantitative importance). Typical examples for these 4 quantitative profiles are shown in the right panel