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Fig. 5 | BMC Genomics

Fig. 5

From: Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

Fig. 5

New annotations for Olfr1508 and Olfr1509 3’UTR isoforms and experimental validation. a RNA-Seq raw data within a 11.7-kb region comprising both Olfr1508 and Olf1509 3’UTRs (see Fig. 1b for legend). Junction coverage is shown for the Olfr1508 strand. b 3’UTRs identified using our IsoSCM pipeline processing the male_IS2014 dataset. Black vertical box: CDS end; black horizontal bar: 3’UTR. c In silico validation of the 3’UTRs annotated using our IsoSCM pipeline by the presence of canonical PASs. Vertical bar = predicted canonical (AAUAAA or AUUAAA) PAS. d Experimental validation by RL-PAT. ✔ = genuine polyA site. The optional splicing in the 3’M of Olfr1508 has been confirmed by 3’RACE (3′ Rapid Amplification of cDNA ends). S: short, M: medium

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