Skip to main content

Table 2 Oligonucleotides used in RL-PAT experiments

From: Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

General primers Primer sequence   
 P1 5′-GGTCACCTTGATCTGAAGC-3’   
 P1’ 5′-GCTTCAGATCAAGGTGACCTTTTT-3’   
Gene specific primers Forward primer sequence (P2) PolyA site tested Position (nt after stop)
 Olfr1507 5′-TGACATGACAACATTTCATTCTGA-3’ XS 69
5′-ATCTGTATTCACTCAAAGAGTTTAGAGTTT-3’ S 1224
5′-GATCTCCTGAGCCATCAACTATCA-3’ M 1986
5′-TGGGCCTTCCTAAATTCTTAAATA-3’ L 3993
 Olfr15 5′-ATCCAGCAACTGGTTTAGAACAAA-3’ S 788
5′-AAGCTGGGGTTACAACTCAGTGAT-3’ M2 2698
 Olfr1508 5′-TACTGTTTGCAATTGTGTGAATTT-3’ XS 2586
5′-TGTAACACCTTGAATAGCTCAAAA-3’ S 2915
5′-TCAAAGGGATTCAATAACTTCTTC-3’ M 4133
 Olfr1509 5′-GTGCTTGTGTGATGTTGTGATAA-3’ XS 15
5′-CTAGGTGTATAATGTTTGGGGTTT-3’ S 289
5′-TGTTTTACTATCCATTGGCTTGAT-3’ 348
5′-CGATAGACAACTTGGAAGAGAACT-3’ M 1086
5′-CTATCGTTCCAGTTAGGACTTCAC-3’ L 4347
 Olfr16 5′-ACATGCTCATCAAATACGGTGTCT-3’ S 69
5′-AAAGGCCCCACATTTCATTATTTA-3’ M 498
  1. The general primers consist in a synthetic sequence P1 and its reverse complement P’1 with a (T)5 anchor [90]