Alternative polyadenylation produces multiple 3’ untranslated regions of odorant receptor mRNAs in mouse olfactory sensory neurons

Table 2 Oligonucleotides used in RL-PAT experiments

General primers	Primer sequence
P1	5′-GGTCACCTTGATCTGAAGC-3’
P1’	5′-GCTTCAGATCAAGGTGACCTTTTT-3’
Gene specific primers	Forward primer sequence (P2)	PolyA site tested	Position (nt after stop)
Olfr1507	5′-TGACATGACAACATTTCATTCTGA-3’	XS	69
	5′-ATCTGTATTCACTCAAAGAGTTTAGAGTTT-3’	S	1224
	5′-GATCTCCTGAGCCATCAACTATCA-3’	M	1986
	5′-TGGGCCTTCCTAAATTCTTAAATA-3’	L	3993
Olfr15	5′-ATCCAGCAACTGGTTTAGAACAAA-3’	S	788
Olfr15	5′-AAGCTGGGGTTACAACTCAGTGAT-3’	M2	2698
Olfr1508	5′-TACTGTTTGCAATTGTGTGAATTT-3’	XS	2586
	5′-TGTAACACCTTGAATAGCTCAAAA-3’	S	2915
	5′-TCAAAGGGATTCAATAACTTCTTC-3’	M	4133
Olfr1509	5′-GTGCTTGTGTGATGTTGTGATAA-3’	XS	15
	5′-CTAGGTGTATAATGTTTGGGGTTT-3’	S	289
	5′-TGTTTTACTATCCATTGGCTTGAT-3’	S	348
	5′-CGATAGACAACTTGGAAGAGAACT-3’	M	1086
	5′-CTATCGTTCCAGTTAGGACTTCAC-3’	L	4347
Olfr16	5′-ACATGCTCATCAAATACGGTGTCT-3’	S	69
Olfr16	5′-AAAGGCCCCACATTTCATTATTTA-3’	M	498

The general primers consist in a synthetic sequence P1 and its reverse complement P’1 with a (T)₅ anchor [90]

ISSN: 1471-2164