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Fig. 2 | BMC Genomics

Fig. 2

From: What makes a bad egg? Egg transcriptome reveals dysregulation of translational machinery and novel fertility genes important for fertilization

Fig. 2

Validation of the microarray data by performance of quantitative real-time PCR (qPCR). Eight genes, including (a) rpf2, (b) spon1b, (c) tspan7b, (d) rps27.2, (e) stra13, (f) rtn4ip1, (g) U1, and (h) slc29a1a were subjected to qPCR using the primers listed in Additional file 2, whereby LSM couples member 14B (lsm14b), prefoldin subunit 2 (pfdn2), and ring finger protein 8 (rnf8) as well as 18S rRNA, beta-actin (bact), and elongation factor 1 alpha (EF1α) were used as internal controls. * p-value ≤0.05, ** p-value ≤0.01, *** p-value ≤0.001, **** p-value<< 0.001

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