Fig. 5

Analysis of transcriptome data for six oviduct samples from the periods before and after foam nest construction in Rhacophorus omeimontis. a. Principal component analysis (PCA) of six oviduct transcriptome data sampled from two periods of foam nest construction. The first principal component (PC1) accounts for 55.48% of the variation, while the second principal component (PC2) accounts for 24.07% of the variation. b. Hierarchical clustering analysis of differentially expressed genes in oviduct between the two periods during foam nest construction. The intensity of colors indicates the expression levels of the genes