Fig. 1

Isolation of Podosphaera xanthii haustorial cells by fluorescence-activated cell sorting. Haustoria were isolated as described in the Methods section, and fungal particles were stained with WGA-Alexa Fluor 488 (green fluorescence). a A CLSM image of a haustorial homogenate before cell sorting showing a haustorial cell (green) and many particles and cell debris including chloroplasts (red). b Counts versus log green fluorescence of particles from haustorial homogenate. The green population (R1) represents fungal particles stained with WGA-Alexa Fluor 488. c Log green fluorescence versus log forward scatter. The red population (R2) indicates selected cells corresponding to haustoria. Events shown as green dots (e.g., hyphal debris or spores) and black (e.g., chloroplasts or plant cell wall debris) were rejected. d A CLSM image showing purified haustoria after fluorescence-activated cell sorting. Bars, 25 μm