Fig. 2

Mapping statistics and read coverage over transcripts for all the libraries prepared with standard input protocols. a The rRNA mapping rate was calculated as the percentage of fragments that were mappable to rRNA sequences. b The non-rRNA mapping rate was calculated from all the non-rRNA fragments as the percentage of fragments with both ends or one end mapped to the genome. c Multiple alignment rates were determined from non-rRNA fragments that were mapped to multiple locations of the genome. d Read-bias was assessed using the read coverage over transcripts. Each transcript was subdivided evenly into 1000 bins and the read coverage was averaged over all the transcripts