Fig. 6

Mapping statistics, read coverage bias, and transcriptome representation for libraries prepared using the SMARTer Ultra Low RNA Kit. a The percentage of fragments mapped to rRNA sequences. b Of all the non-rRNA fragments, the percentage of fragments with both ends or one end mapped to the genome. c The read coverage over transcripts. Each transcript was subdivided evenly into 1000 bins and the read coverage was averaged over all the transcripts. d Composition of the uniquely mapped fragments, shown as the percentage of fragments in exonic, intronic, and intergenic regions. According to the direction of transcription, exonic and intronic regions were further divided to sense and antisense. e Saturation analysis showing the percentage of coding genes recovered at increasing sequencing depth. f Saturation analysis showing the percentage of lncRNAs recovered at increasing sequencing depth. g Saturation analysis showing the number of splice junctions recovered at increasing sequencing depth. For the purpose of evaluation, the above analyses also include the libraries prepared with the TruSeq Stranded mRNA protocol using the same biological conditions