Fig. 5

Physiological effect of the OYE2 alleles. a. Sequence alignments of Saccharomyces cerevisiae Oye2p proteins. The strains F10 (parental strain of G-4A), OS104 and S294 show stop-codon insertion at different positions. b. The bar plots represent the average values of residual sugars after isothermal fermentations carried out at 24, 30 and 32 °C in both hemyzygous hybrids and the native H4 hybrid. The genotypes ΔOYE2^B::KanMx4/OYE2^G, OYE2^B/ΔOYE2^G::KanMx4, and OYE2^B/OYE2^G are shown in red, green and black, respectively. Bars represent standard error of five repetitions, the statistical differences between the hemizygous was tested by a Wilcoxon-Mann-Whitney Test (the p value is coded as follow, ‘ns’ = p > 0.05, ‘.’ = p < 0.1, ‘**’ = p < 0.01. c. Fermentation kinetics (CO₂ produced time course) for the same strains and with the same color key